Mammalian Diabetes
- Rat Insulin ELISA
- Ultrasensitive Rat Insulin ELISA
- High Range Rat Insulin ELISA
- Porcine Insulin ELISA
- Ovine Insulin ELISA
- Bovine Insulin ELISA
- Mouse Insulin ELISA
- Ultrasensitive Mouse Insulin ELISA
- Glucagon 10ul ELISA
- Rat C-peptide ELISA
- Rat/Mouse Proinsulin ELISA
- High Range Porcine insulin ELISA
- Feline Insulin ELISA
- Canine Insulin ELISA
- Equine Insulin ELISA
- Porcine C-peptide ELISA
- Rat Glicentin ELISA
| Description | Rat Insulin ELISA | Ultrasensitive Rat Insulin ELISA | High Range Rat Insulin ELISA |
| Code No. | 10-1250-01 10-1250-10 | 10-1251-01 | 10-1145-01 |
| No. of tests | 96/10x96 | 96 | 96 |
| Measured antigen | Rat/mouse insulin | Rat/mouse insulin | Rat/mouse insulin |
| Standards mU/l | 0, 0.15, 0.4, 1, 3, 5.5 | 0, 0.02, 0.05, 0.15 0.4, 1.0 | 0, 3.0, 7.5, 30.0, 75.0, 150.0 |
| Calibration | |||
| Incubations (mins) | 120+15 | 120+15 | 120+15 |
| Sample/sample volume | serum/plasma 10ul | serum/plasma 25ul | serum/plasma 10ul |
| Sensitivity | ≤0.15ug/l | 1) ≤0.02ug/l | 1.5 ug/l/ 263 pmol/l |
| Precision intra/inter cv% | <5.2%, <10.1% | 1) <3% 1) <5% | <5%/<5% |
| Hook | >450 ug/l | 450 ug/l | 576 ug/l 100.8nmol/l |
| Cross-reactions | - Human insulin 167% - Insulin Lispro (Humalog®) 167% - Human proinsulin 75% - Human C-peptide <0.05% - IGF-I <0.02% - IGF-II <0.02% - Rat C-peptide <0.001% - Rat proinsulin 7% - Mouse insulin 75% - Porcine insulin 476% - Sheep insulin 179% - Bovine insulin 78% - No interference by lipaemic, icteric or haemolysed samples | - insulin (mouse) 81% - insulin (human) 120% - insulin (Humalog®)120% - proinsulin (human) 71% - C-peptide (human) <0.05% - No interference by lipaemic, icteric or haemolysed samples | - insulin (mouse) 81% - insulin (human) 120% - insulin (Humalog®) 120 - proinsulin (human) 71% C-peptide (human)<0.05% - No interference by lipaemic, icteric or haemolysed samples |
| Description | Porcine Insulin ELISA | Ovine Insulin ELISA | Bovine Insulin ELISA |
| Code No. | 10-1200-01 | 10-1202-01 | 10-1201-01 |
| No. of tests | 96 | 96 | 96 |
| Measured antigen | Porcine insulin | Ovine insulin | Bovine insulin |
| Standards mU/l | 0, 0.02, 0.05, 0.15, 0.5, 1.5 | 0, 0.05, 0.15, 0.5, 1.5, 3.0 | 0, 0.05, 0.15, 0.5, 1.5, 3.0 |
| Calibration | in-house reference | in-house reference | in-house reference |
| Incubations (mins) | 120+15 | 120+15 | 120+15 |
| Sample/sample volume | Serum/plasma 25ul | Serum/plasma 25ul | Serum/plasma 25ul |
| Sensitivity | ≤0.01ug/l | ≤0.025ug/l | ≤0.025ug/l |
| Precision intra/inter cv% | <3.8% <3.9% | <3.7% <6.5% | <5.3% <7.0% |
| Hook | >1000ug/l | >1000ug/l | >1000ug/l |
| Cross-reactions | No interference by lipaemic, icteric or haemolysed samples | No interference by lipaemic, icteric or haemolysed samples | No interference by lipaemic, icteric or haemolysed samples |
| Description | Mouse Insulin ELISA | Ultrasensitive Mouse Insulin ELISA | Glucagon 10ul ELISA |
| Code No. | 10-1247-01 10-1247-10 | 10-1249-01 | 10-1281-01 |
| No. of tests | 96/10x96 | 96 | 96 |
| Measured antigen | Mouse Insulin | Mouse Insulin | Glucagon. validated for rat, mice, porcine & non-human primate. |
| Standards mU/l | 0, 0.188, 0.5, 1.25, 3.75, 6.9 | 0, 0.025, 0.063, 0.188, 0.5, 1.25 | |
| Incubations (mins) | 120+15 | 120+15 | overnight+15 |
| Sample/sample volume | serum/plasma 10ul | serum/plasma 25ul | Serum/plasma/cell culture |
| Sensitivity | ≤0.2 ug/l | ≤0.025 ug/l | 1.5 pmol/l |
| Precision intra/inter cv% | <3.2%/<6% | <3%/<5% | Total 8.3 17.9% |
| Hook | >450 ug/l | 450 ug/l | >8 umol/l |
| Cross-reactions | - Human insulin 167% No interference by lipaemic, icteric or haemolysed samples | No interference by lipaemic, icteric or haemolysed samples | - Oxyntomodulin, human, rat, mouse 2.0% - Oxyntomodulin, bovine, canine, porcine n.d. - Mini-Glucagon n.d. - GLP-1 n.d. - GLP-2 n.d. - Glicentin, human 1.0% - Glicentin, mouse 7.0% - Glicentin, rat 4.0% |
| Description | Rat C-peptide ELISA | Rat/Mouse proinsulin ELISA |
| Code No. | 10-1172-01 | 10-1232-01 |
| No. of tests | 96 | 96 |
| Measured antigen | Rat C-peptide | Rat and Mouse proinsulin |
| Standards mU/l | c. 0 - 4000 (0 + 5 standards) | c. 0 - 2000 (0 + 5 standards) |
| Incubations (mins) | 60 + 60 + 15 | 120+30 |
| Sample/sample volume | serum/plasma 10ul | serum/plasma 25ul |
| Sensitivity | %td 3 pmol/l | |
| Precision intra/inter cv% | <4.3%/<7.3% | <3.7%/<6.4% |
| Hook | 400000pmol/l | 90000pmol/l |
| Cross-reactions | - Human C-peptide <0.001% - Rat proinsulin 4.55% - Rat insulin <0.01% - No interference by lipaemic, icteric or haemolysed samples | - Human insulin <0.0000001% - Human proinsulin 2 - 10% (mean 5%) - Human C-peptide <0.000001% - Rat C-peptide <0.0000001% - Rat insulin <0.000004% - Mouse insulin <0.0003% - Mouse C-peptide <0.00007% - No interference by lipaemic, icteric or haemolysed samples |
| Description | High Range Porcine Insulin ELISA | Feline Insulin ELISA |
| Code No. | 10-1223-01 | 10-1233-01 |
| No. of tests | 96 | 96 |
| Measured antigen | Porcine Insulin | Feline Insulin |
| Standards mU/l | c. 0 - 75 g/l (0 + 5 standards) | 0, 5, 35, 100, 350, 700 ng/l |
| Incubations (mins) | 60 + 15 | 120+30 |
| Sample/sample volume | serum/plasma 10ul | serum/plasma 10ul |
| Sensitivity | 0.313ug/l | 5ng/l |
| Precision intra/inter cv% | <2.3%/<5% | <4.8%/<8.9% |
| Hook | 11500 ug/l | 27600 ug/l |
| Cross-reactions | - Porcine C-peptide <0.001% - Porcine Proinsulin <0.2% - Human insulin 28% - Human Proinsulin <0.1% - Human C-peptide <0.01% - Aspart (NovoRapid) 0.7% - Detemir (Levemir) <0.0000002% - Glargin (Lantus) 5.4% - Lispro (Humalog) <0.0000006% - No interference by lipaemic, icteric or haemolysed samples | - Aspart (NovoRapid®) 5.9% - Detemir (Levemir®) <0.008% - Glargin (Lantus®) 8.4% - Lispro (Humalog®) <0.00000002% - Glulisine (Apidra®) <0.0000007% - Letsulin® , Caninsulin® 57.4% - No interference by lipaemic, icteric or haemolysed samples |
| Description | Canine Insulin ELISA | Equine Insulin ELISA |
| Code No. | 10-1203-01 | 10-1205-01 |
| No. of tests | 96 | 96 |
| Measured antigen | Canine Insulin | Equine Insulin |
| Standards mU/l | 0, 0.02, 0.05, 0.15, 0.50, 1.50 | 0, 0.02, 0.05, 0.15, 0.50, 1.50 |
| Incubations (mins) | 120 + 15 | 120 + 15 |
| Sample/sample volume | serum/plasma 25ul | serum/plasma 25ul |
| Sensitivity | ≤0.01 ug/l | ≤0.01 ug/l |
| Precision intra/inter cv% | <4.5%/<6.2% | <3.4%/<4.3% |
| Hook | >1000ug/l | >1000ug/l |
| Cross-reactions | - porcine insulin 100% - porcine C-peptide <0.001% - porcine proinsulin <0.20% - human insulin 28% - human C-peptide <0.01% - human proinsulin <0.1% - NovoRapid 0.7% - Levemir <0.0000002% - Lantus 5.4% - Humalog <0.0000006% - No interference by lipaemic, icteric or haemolysed samples | - porcine insulin 100% - porcine C-peptide <0.001% - porcine proinsulin <0.20% - human insulin 28% - human C-peptide <0.01% - human proinsulin <0.1% - NovoRapid 0.7% - Levemir <0.0000002% - Lantus 5.4% - Humalog <0.0000006% - No interference by lipaemic, icteric or haemolysed samples |
| Description | Porcine C-peptide ELISA | Rat Glicentin ELISA |
| Code No. | 10-1156-01 | 10-1275-01 |
| No. of tests | 96 | 96 |
| Measured antigen | Porcine C-peptide | Rat Glicentin |
| Standards mU/l | c. 0—1500 (0 + 5 standards) | 5—500 pmol/l |
| Incubations (mins) | 120 + 60 + 15 | overnight + 15 |
| Sample/sample volume | serum/plasma/cell culture 10ul | Serum/plasma 10ul |
| Sensitivity | 10 pmol/L | 5 pmol/L |
| Precision intra/inter cv% | <3.7%/<3.8% | Total 7—14% |
| Hook | >30000 pmol/L | >11,000 pmol/l |
| Cross-reactions | - Porcine insulin <0.05% - Porcine proinsulin <0.02% - Human C-peptide <0.003% - Human proinsulin <0.01% - Macaque C-peptide <0.006% - Mouse C-peptide <0.007% - Mouse insulin <0.13% - Mouse proinsulin <0.002% - Rat C-peptide 0.08% - Rat insulin <0.002% - Rat proinsulin <0.002% | - Oxyntomodulin ND - Mini-Glucagon ND - GLP-1 ND - GLP-2 ND - Glucagon ND - Human Glicentin <1.7% - Mouse Glicentin 5.2% |