Human Diabetes
| Description | Insulin ELISA | Iso-Insulin ELISA | Ultrasensitive Insulin ELISA |
| Code No. | 10-1113-01 10-1113-10 | 10-1128-01 | 10-1132-01 |
| No. of tests | 96/10x96 | 96 | 96 |
| Measured antigen | human insulin | human insulin insulin lispro | human insulin |
| Standards mU/l | 0, 3, 10, 30,100, 200 | 0, 3, 10, 30,100 | 0, 0.15, 1, 3, 10, 20 |
| Calibration | 1st IRP 66/304 | 1st IRP 66/304 | 1st IRP 66/304 |
| Incubations (mins) | 60+15 | 60+15 | 60+30 |
| Sample/sample volume | serum/plasma 25ul | serum/plasma 25ul | serum/plasma 25ul |
| Sensitivity | <1 mU/L <6 pmol/L | <1 mU/L <6 pmol/L | <0.07 mU/L <0.42 pmol/L |
| Precision intra/inter cv% | <4%, <4% | <4%, <4% | <6%, <4% |
| Hook | 30000mU/L 180nmol/L | 2000 mU/L 12 nmol/L | 800000 mU/l 4800 nmol/l |
| Cross-reactions | - C-peptide <0.01% - proinsulin <0.01% - insulin lispro <0.006% - h-proinsulin des (64-65) 98% - h-proinsulin split (65-66) 56% - h-proinsulin des (31-32) <0.5% - h-proinsulin split (32-33) <0.5% | - C-peptide <0.1% - proinsulin 54% - insulin lispro 100% - h-proinsulin des (64-65) 66% - h-proinsulin split (65-66) 78% - h-proinsulin des (31-32) 58% - h-proinsulin split (32-33) 56% | - C-peptide <0.01% - proinsulin <0.01% - insulin lispro <0.006% - h-proinsulin des (64-65) 98% - h-proinsulin split (65-66) 56% - h-proinsulin des (31-32) <0.5% - h-proinsulin split (32-33) <0.5% |
| Description | C-peptide ELISA specific | Ultrasensitive C-peptide ELISA | Proinsulin ELISA |
| Code No. | 10-1136-01 | 10-1141-01 | 10-1118-01 |
| No. of tests | 96 | 96 | 96 |
| Measured antigen | human C-peptide | human C-peptide | human proinsulin |
| Standards mU/l | 0, 100, 330, 1300, 2500, 4000 | 0, 5, 16, 60, 120, 230 | 0, 3.3, 11, 44, 132 |
| Calibration | IRR 84/510 | IRR 84/510 | IRR 84/611 |
| Incubations (mins) | 60+60+15 | 60+60+30 | 60+60+15 |
| Sample/sample volume | serum/plasma 25ul | serum/plasma 25ul | serum/plasma 50ul |
| Sensitivity | ≤15pmol/L ≤0.045ug/L | <1.5 pmol/L ≤0.0045ug/L | ≤0.5 pmol/L ≤0.0045ug/L |
| Precision intra/inter cv% | <5%, <5% | <4%, <6% | <5%, <6% |
| Hook | 1750 nmol/L | 36.6 nmol/L | 80 nmol/lq |
| Cross-reactions | - Insulin <0.0006% - proinsulin <1.8% - h-proinsulin des (64-65) 74% - h-proinsulin split (65-66) 10% - h-proinsulin des (31-32) 3% - h-proinsulin split (32-33) 2% | - Insulin <0.0006% - proinsulin 3% - h-proinsulin des (64-65) 74% - h-proinsulin split (65-66) 10% - h-proinsulin des (31-32) 3% - h-proinsulin split (32-33) 2% | - Insulin <0.03% - C-peptide <0.006% - h-proinsulin des (64-65) 84% - h-proinsulin split (65-66) 90% - h-proinsulin des (31-32) 95% - h-proinsulin split (32-33) 95% |
| Description | Leptin ELISA | Insulin Pre-Transplantation ELISA |
| Code No. | 10-1199-01 | 10-1222-01 |
| No. of tests | 96 | 96 |
| Measured antigen | human leptin | human insulin |
| Standards mU/l | 0, 0.10, 0.25, 0.65, 1.50, 5.0 ng/ml | 0, 3.0, 10.0, 30.0, 100.0, 200.0, mU/l |
| Calibration | 1st IS NIBSC Code 97/594 | 1st IRP 66/304 |
| Incubations (mins) | 120+15 | 15+15 |
| Sample/sample volume | serum/plasma diluted 1/21 | serum/plasma |
| Sensitivity | 0.05 ng/ml | 1.5 mU/l |
| Precision intra/inter cv% | <3% <5.2% | <3% <4.5% |
| Hook | >100,000 ng/ml | |
| Cross-reactions | - CNTF ≤0.05% - G-CSF ≤0.0004% - IL-6 ≤0.0002% - IL-11 ≤0.0008% - IL-12 ≤0.0004% - LIF ≤0.002% - Oncostatin ≤0.0002% - Rat Leptin 0.02% - Mouse Leptin ≤0.001% - Sheep Leptin 0.003% | - C-peptide <0.01% - Proinsulin <0.01% - Proinsulin des (64-65) 98% - Proinsulin split (65-66) 56% - Proinsulin des (31-32) <0.5% - Proinsulin split (32-33) <0.5% - Insulin Lispro (Humalog®, Eli Lilly) <0.006% - Insulin aspart <0.006% - IGF-I <0.02% - IGF-II <0.02% - Rat insulin 0.7% - Mouse insulin 0.3% - Porcine insulin 374% - Sheep insulin 48% - Bovine insulin 31% |
| Description | Glucagon ELISA | Glicentin ELISA | GLP-1 NL-ELISA |
| Code No. | 10-1271-01 | 10-1273-01 | 10-1278-01 |
| No. of tests | 96 | 96 | 96 |
| Measured antigen | Human Glucagon | Human Glicentin | Amidated GLP-1 |
| Measuring range | 1.5—120 pmol/l | 3—300 pmol/l | 0.9 -940 pmol/l (2.8 - 2900 pg/ml) |
| Calibration | WHO 1st IRP 69/194 | in-house | Against a highly purified, fully validated commercial preparation |
| Incubations (mins) | overnight+15 | 60+60+15 | 120+15 |
| Sample/sample volume | Serum/plasma/cell culture 25ul | Serum/plasma/cell culture 25ul | 25ul |
| Sensitivity | 1 pmol/l | 3 pmol/l | 0.65 pmol/l |
| Precision intra/inter cv% | Total 7.5—9.5% | Total 6—12.2% | 2.5—11 |
| Hook | >8 umol/l | >100 nmol/l | >18.8 nmol/L |
| Cross-reactions | - Oxyntomodulin 4.4% - Mini-Glucagon <0.1% - GLP-1 <0.3% - GLP-2 <0.3% - Glicentin 0.8% | - Oxyntomodulin ND - Mini-Glucagon ND - GLP-1 ND - GLP-2 ND - Mouse Glicentin ND - Glucagon ND - Rat Glicentin 0.9% | - GLP-1 (1-36) amide 88% - GLP-1 (7-36) amide 93% - GLP-1 (9-36) amide 100% - others - see Directions for use |
| Description | Lispro NL_ELISA |
| Code No. | 10-1291-01 |
| No. of tests | 96 |
| Measured antigen | Insulin Lispro |
| Measuring range | 1 - 500 mU/l |
| Calibration | EuPh Ref: Insulin Lispro CRS4 |
| Incubations (mins) | 60 + 60 + 10 |
| Sample/sample volume | Serum/plasma 10ul |
| C of D/LLOQ | 0.36 mU/l 1.0 mU/l |
| Precision intra/inter cv% | Total 3.0—7.7% |
| Hook | None up to 10,000 mU/l |
| Cross-reactions | The following were not dectected: - Native human insulin - Native human proinsulin - Glargine - Glargine M1 - Glargine M2 - Degludec - Detemir - Insulin NPH - Aspart - Glulisine - Native Porcine insulin - Native Porcine proinsulin |