DescriptionInsulin ELISAIso-Insulin ELISAUltrasensitive Insulin ELISA
Code No.10-1113-01
10-1113-10
10-1128-0110-1132-01
No. of tests96/10x96 9696
Measured antigenhuman insulinhuman insulin insulin lisprohuman insulin
Standards mU/l0, 3, 10, 30,100, 2000, 3, 10, 30,1000, 0.15, 1, 3, 10, 20
Calibration1st IRP 66/3041st IRP 66/3041st IRP 66/304
Incubations (mins)60+1560+1560+30
Sample/sample volumeserum/plasma 25ulserum/plasma 25ulserum/plasma 25ul
Sensitivity<1 mU/L <6 pmol/L<1 mU/L <6 pmol/L<0.07 mU/L
<0.42 pmol/L
Precision intra/inter cv%<4%, <4%<4%, <4%<6%, <4%
Hook30000mU/L
180nmol/L
2000 mU/L
12 nmol/L
800000 mU/l
4800 nmol/l
Cross-reactions- C-peptide <0.01%
- proinsulin <0.01%
- insulin lispro <0.006%
- h-proinsulin des (64-65) 98%
- h-proinsulin split (65-66) 56%
- h-proinsulin des (31-32) <0.5%
- h-proinsulin split (32-33) <0.5%
- C-peptide <0.1%
- proinsulin 54%
- insulin lispro 100%
- h-proinsulin des (64-65) 66%
- h-proinsulin split (65-66) 78%
- h-proinsulin des (31-32) 58%
- h-proinsulin split (32-33) 56%
- C-peptide <0.01%
- proinsulin <0.01%
- insulin lispro <0.006%
- h-proinsulin des (64-65) 98%
- h-proinsulin split (65-66) 56%
- h-proinsulin des (31-32) <0.5%
- h-proinsulin split (32-33) <0.5%

DescriptionC-peptide ELISA specificUltrasensitive C-peptide ELISAProinsulin ELISA
Code No.10-1136-0110-1141-0110-1118-01
No. of tests969696
Measured antigenhuman C-peptidehuman C-peptidehuman proinsulin
Standards mU/l0, 100, 330, 1300, 2500, 40000, 5, 16, 60, 120, 2300, 3.3, 11, 44, 132
CalibrationIRR 84/510IRR 84/510IRR 84/611
Incubations (mins)60+60+1560+60+3060+60+15
Sample/sample volumeserum/plasma 25ulserum/plasma 25ulserum/plasma 50ul
Sensitivity≤15pmol/L
≤0.045ug/L
<1.5 pmol/L
≤0.0045ug/L
≤0.5 pmol/L
≤0.0045ug/L
Precision intra/inter cv%<5%, <5%<4%, <6%<5%, <6%
Hook1750 nmol/L36.6 nmol/L80 nmol/lq
Cross-reactions- Insulin <0.0006%
- proinsulin <1.8%
- h-proinsulin des (64-65) 74%
- h-proinsulin split (65-66) 10%
- h-proinsulin des (31-32) 3%
- h-proinsulin split (32-33) 2%
- Insulin <0.0006%
- proinsulin 3%
- h-proinsulin des (64-65) 74%
- h-proinsulin split (65-66) 10%
- h-proinsulin des (31-32) 3%
- h-proinsulin split (32-33) 2%
- Insulin <0.03%
- C-peptide <0.006%
- h-proinsulin des (64-65) 84%
- h-proinsulin split (65-66) 90%
- h-proinsulin des (31-32) 95%
- h-proinsulin split (32-33) 95%

DescriptionLeptin ELISAInsulin Pre-Transplantation ELISA
Code No.10-1199-0110-1222-01
No. of tests9696
Measured antigenhuman leptinhuman insulin
Standards mU/l0, 0.10, 0.25, 0.65, 1.50, 5.0 ng/ml0, 3.0, 10.0, 30.0, 100.0, 200.0, mU/l
Calibration1st IS NIBSC Code 97/5941st IRP 66/304
Incubations (mins)120+1515+15
Sample/sample volumeserum/plasma diluted 1/21serum/plasma
Sensitivity0.05 ng/ml1.5 mU/l
Precision intra/inter cv%<3% <5.2%<3% <4.5%
Hook>100,000 ng/ml
Cross-reactions- CNTF ≤0.05%
- G-CSF ≤0.0004%
- IL-6 ≤0.0002%
- IL-11 ≤0.0008%
- IL-12 ≤0.0004%
- LIF ≤0.002%
- Oncostatin ≤0.0002%
- Rat Leptin 0.02%
- Mouse Leptin ≤0.001%
- Sheep Leptin 0.003%
- C-peptide <0.01%
- Proinsulin <0.01%
- Proinsulin des (64-65) 98%
- Proinsulin split (65-66) 56%
- Proinsulin des (31-32) <0.5%
- Proinsulin split (32-33) <0.5%
- Insulin Lispro (Humalog®, Eli Lilly) <0.006%
- Insulin aspart <0.006%
- IGF-I <0.02%
- IGF-II <0.02%
- Rat insulin 0.7%
- Mouse insulin 0.3%
- Porcine insulin 374%
- Sheep insulin 48%
- Bovine insulin 31%

DescriptionGlucagon ELISAGlicentin ELISAGLP-1 NL-ELISA
Code No.10-1271-0110-1273-0110-1278-01
No. of tests969696
Measured antigenHuman GlucagonHuman GlicentinAmidated GLP-1
Measuring range1.5—120 pmol/l3—300 pmol/l 0.9 -940 pmol/l (2.8 - 2900 pg/ml)
CalibrationWHO 1st IRP 69/194in-house Against a highly purified, fully validated commercial preparation
Incubations (mins)overnight+1560+60+15120+15
Sample/sample volumeSerum/plasma/cell culture 25ulSerum/plasma/cell culture 25ul25ul
Sensitivity1 pmol/l3 pmol/l0.65 pmol/l
Precision intra/inter cv%Total 7.5—9.5%Total 6—12.2%2.5—11
Hook>8 umol/l>100 nmol/l>18.8 nmol/L
Cross-reactions- Oxyntomodulin 4.4%
- Mini-Glucagon <0.1%
- GLP-1 <0.3%
- GLP-2 <0.3%
- Glicentin 0.8%
- Oxyntomodulin ND
- Mini-Glucagon ND
- GLP-1 ND
- GLP-2 ND
- Mouse Glicentin ND
- Glucagon ND
- Rat Glicentin 0.9%
- GLP-1 (1-36) amide 88%
- GLP-1 (7-36) amide 93%
- GLP-1 (9-36) amide 100%
- others - see Directions for use

DescriptionLispro NL_ELISA
Code No.10-1291-01
No. of tests96
Measured antigenInsulin Lispro
Measuring range1 - 500 mU/l
CalibrationEuPh Ref: Insulin Lispro CRS4
Incubations (mins)60 + 60 + 10
Sample/sample volumeSerum/plasma 10ul
C of D/LLOQ0.36 mU/l 1.0 mU/l
Precision intra/inter cv%Total 3.0—7.7%
HookNone up to 10,000 mU/l
Cross-reactionsThe following were not dectected:

- Native human insulin
- Native human proinsulin
- Glargine
- Glargine M1
- Glargine M2
- Degludec
- Detemir
- Insulin NPH
- Aspart
- Glulisine
- Native Porcine insulin
- Native Porcine proinsulin